One shot mode Continuous mode
Focusing position Dry objective Interface between air and cover glass
Immersion objective Interface between sample
(cultured liquid) and cover glass
Offset method controlled by software Compensation for shift of observation position toward the focusing plane is by Z-axis control
Observation methods Brightfield, DIC and fluorescence Brightfield and fluorescence
FN limitation Light volume is low at the image perimeter for F.N. 22 when using 2x, 4x, 10x objectives
Focusing speed Depending on software implementation Sampling rate 10ms
Focusing accuracy Dry objective Standard deviation: σ =0,1µm
Immersion objective Standard deviation: σ =0,07µm
Laser Laser diode 785nm, 5µW